Journal: The Journal of Experimental Medicine

Article Title: Tet2 deficiency–induced expansion of monocyte-derived macrophages promotes liver fibrosis

doi: 10.1084/jem.20251114

Figure Lengend Snippet: Effect of Tet2 deficiency on Ccl2 and Ccl8 mRNA stability and IL-6 neutralization on liver fibrosis progression. (A) The frequency of Ccr2 + and Ccr3 + pMDMs in Tet2 WT -CCl 4 and Tet2 ΔMye -CCl 4 mice ( n = 4 for each group). (B) Expression of Ccr2 and Ccr3 on monocytes, Tet2 +/+ pMDMs, and Tet2 −/− pMDMs ( n = 4 for each group). (C) The infiltration difference of other Ccr2 or Ccr3 expressing cell types in Tet2 WT -CCl 4 and Tet2 ΔMye -CCl 4 mice ( n = 4 for each group). (D) GAPDH mRNA decay curve in Tet2 +/+ pMDMs and Tet2 −/− pMDMs ( n = 6 for each group). (E) Transcriptional level of Elavl1, Znf36, and Ybx1 in Tet2 +/+ pMDMs and Tet2 −/− pMDMs ( n = 4 for each group). (F–I) Serum levels of (F) IL-1α, (G) IL-2, (H) TNFα, and (I) IL-1β in livers of Tet2 WT -CCl 4 and Tet2 ΔMye -CCl 4 mice ( n = 5 for each group). (J) Serum IL-6 levels in scramble, WT-MT, and KO-MT mice ( n = 4 for each group). (K) Il-6 levels in CD45.2 + pMDMs isolated from livers of WT-MT and KO-MT mice ( n = 4). (L) mRNA levels of Acta2 and Col1a1 in Tet2 +/+ and Tet2 −/− HSCs ( n = 4 for each group). (M and N) Effect of anti–IL-6 Abs treatment on mRNA levels of Col1a1 (M) and Acta2 (N) in Tet2 +/+ and Tet2 −/− HSCs co-cultured with Tet2 +/+ or Tet2 −/− MDMs detected by RT-PCR in vitro ( n = 3 for each group). (O) Effect on recombinant Ccl2 and Ccl8 on Il-6 expression in Tet2 +/+ pMDMs and Tet2 −/− pMDMs ( n = 3 for each group). (P) Detection of MDMs in livers by flow cytometry after Bindarit or IL-6 Abs treatment for 2 wk ( n = 5 for each group). (Q) H&E staining of liver tissues treated with PBS, Bindarit, IL-6 Abs, or Bindarit plus IL-6 Abs in Tet2 WT -CCl 4 and Tet2 ΔMye -CCl 4 mice ( n = 4 for each group). Data are representative of at least two independent experiments with similar results (A–P). All data are shown as mean ± SD and were analyzed by two-tailed, unpaired Student’s t test (A, C, D, E, K, L, and O) or one-way ANOVA with Tukey’s multiple comparison test (B and J) or two-way ANOVA with Sidak’s multiple comparison test (F–I, M, and N). ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05; P > 0.05 not significant (ns).

Article Snippet: ELISA was used to detect the levels of Col IV (#20024; Ruixin Biotechnology), HA (#20067; Ruixin Biotechnology), Ccl8 (#RX27820; Ruixin Biotechnology), Ccl2 (MG9180; Fantia), TNFα (#KE10002; Proteintech), IL-2 (#BGM4904; Bangjing), IL-1α (KE10098; Proteintech), IL-1β (KE10003; Proteintech), and IL-6 (#KE10091; Proteintech) in serum and liver tissue grinding fluid.

Techniques: Neutralization, Expressing, Isolation, Cell Culture, Reverse Transcription Polymerase Chain Reaction, In Vitro, Recombinant, Flow Cytometry, Staining, Two Tailed Test, Comparison

Journal: Neural Regeneration Research

Article Title: Sox2-overexpressing neural stem cells alleviate ventricular enlargement and neurological dysfunction in posthemorrhagic hydrocephalus

doi: 10.4103/NRR.NRR-D-24-01491

Figure Lengend Snippet: NSC Sox2 transplantation decreases the expression of proinflammatory factors and increases the expression of anti-inflammatory factors and neurotrophic factors in hippocampus. (A, B) ELISA results of TNF-α (A) and IL-1β (B) in hippocampus. (C) Typical western blot bands of TNF-α, IL-6, IL-10, BDNF, and NGF. (D) Statistical analysis of proteins. All data are presented as the mean ± SD ( n = 5). ** P < 0.01, *** P < 0.001, vs . Sham group (one-way analysis of variance followed by post hoc Tukey’s honestly significant difference test). BDNF: Brain-derived growth factor; ELISA: enzyme-linked immunosorbent assay; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IL: interleukin; IVH: intraventricular hemorrhage; NGF: nerve growth factor; NSC: neural stem cell; PBS: phosphate-buffered saline; SD: standard deviation; Sox2: sex-determining region Y-box 2; TNF-α: tumor necrosis factor-alpha.

Article Snippet: The supernatant was collected for the assay using enzyme-linked immunosorbent assay (ELISA) kits (EK0527, EK0394, Boster Bio, Anaheim, CA, USA).

Techniques: Transplantation Assay, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Derivative Assay, Saline, Standard Deviation